Room F248

This inverted microscope is suitable for live cell imaging in 3 and 4D, bleaching (FRAP), photo activation techniques and spectral analyses. It is equipped with a diod laser 405 nm, an Argon laser with 458/488/514 nm lines, DPSS 561 nm and HeNe 633 nm laser. The instrument has a spectral detector (GaAsp 32-channels) for acquisition of Lambda Stacks and subsequent digital separation into component dyes, three additional PMTs, two for confocal detection and one for non-confocal bright field images. The spectral detection unit is adjustable within 390-750 nm. The instrument can be used for FCS and RICS. Standard equipment for ocular observation of reflected light is present as well as a heating stage and an incubator for controlled temperature and CO2-concentration.

The following lenses are available:
Plan-Apo 10x/0.45
Plan-Apo 20x/0.8
Plan-Apo 40x/1.3 Oil
Plan-Apo 63x/1.4 Oil
LD C-Apo 63x/1.15 W corr

The system is controlled by the ZEN black 2011 software.
For image processing a separate workstation with identical software is available.
 

Room F227

This microscope is suitable for fixed samples, 3- and 4D imaging, bleaching and spectral analyses. It is an inverted microscope equipped with an Argon laser (458/477/488/514 nm) a HeNe1 laser (543 nm) and a HeNe2 laser (633 nm). The instrument has a Meta detector (A 32-channel detector) for acquisition of Lambda Stacks and subsequent digital separation into component dyes, two additional PMT’s for confocal detection and one for non confocal bright field images.
 

A standard equipment for ocular observation of reflected light is present.
The following lenses are available:

Plan-Apo 10x/0.45
Plan-Apo 20x/0.8
Plan-Apo 40x/1.0 Oil Ph3
Plan-Apo 63x/1.4 Oil Ph3
Plan-Apo 63x/1.2 W corr
Plan-Apo 100x/1.4 Oil
The system is run either by the Zeiss AIM 4.2 or the ZEN 2007 software. 

Room F235

This inverted microscope is suitable for fixed samples and 3-D imaging. It is equipped with an Argon laser (458 nm, 488 nm,) a HeNe1 laser (543 nm) and a HeNe2 laser (633 nm). It has three PMT’s for confocal detection and one for non confocal brightfield images. A standard equipment for ocular observation of reflected light is present.

The following lenses are available:
Plan-Apo 10x/0.45
Plan-Apo 20x/0.6
Plan-Apo 40x/1.0 Oil Ph3
Plan-Apo 63x/1.4 Oil Ph3
The system is run by the Zeiss AIM 4.2 software.

Room F455

An inverted microscope that is suitable for fixed samples, 3- and 4D live cell imaging (simultaneously at dual channels), bleaching techniques and microinjection experiments. The system has a climate hood for temperature and CO2 control and a heating stage for variable sized culture chambers. In addition there is a femtojet microinjection equipment from Eppendorf. The UV-light source is a Lambda DG-4 illumination system with external excitation filters. There are two digital cameras, AxioCam MRc with an extra emission filter wheel and a Roper Cascade 1K. The latter is coupled to a dual viewer for simultaneous detection of two channels. An external shutter for brightfield recording enables very fast acquisition of both fluorescence and bright field. Additional triggering signals can be added and steered by a TTL unit.

The following lenses are available:
EC-Plan-Neofluar 10x/0.3 Ph1
Plan-Neofluar 16x/0.5 Imm
EC-Plan-Neofluar 40x/ 0.75 Ph2
LCI Plan-Neofluar 63x/1.3 IMM corr (water or glycerol) Ph3
63x/1.4 oil DIC

The system has two optional soft wares for acquisition and image processing, Axiovision and Slide book.
A separate work station with Axiovision, Slide book and AutoQuant (for deconvolution) is available nearby.

Room F227

An inverted microscope suitable for colour photos of histological staining in bright field mode as well as for fluorescent samples. The microscope is equipped with a colour camera, and a mercury lamp and filters for UV (Dapi), blue light (FITC/GFP) and green light (TRITC/Ds Red). Some of the objective lenses are specially designed for imaging through plastic and thick specimens (by modulation contrast).

The following lenses are available:
N-Plan 10x/0.25 Ph1
C-Plan L 20x/0.3 Ph1
N-Plan L 20x/0.4 corr LMC (for modulation contrast)
N-Plan L 40x/0.55 corr LMC (for modulation contrast)

 Room F 256

The microscope is a TECNAI G2 Spirit Bio TWIN (FEI Company) 120 kV with a Tungsten filament. It is a high contrast, general purpose instrument that is specifically suited for low contrast samples. It enables exploration of the native state of low contrast, beam sensitive biological specimens, or other soft materials like polymers. It has a BioTWIN low-background single-tilt holder and a Gatan 70° cryo transfer holder. There are two digital cameras: a SIS MegaView III (in-column) and a Gatan US 1000P (under the column).