Profiles

David Drew

David Drew

Docent

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Works at Department of Biochemistry and Biophysics
Telephone 08-16 22 95
Email david.drew@dbb.su.se
Visiting address Svante Arrhenius väg 16
Room A553
Postal address Institutionen för biokemi och biofysik 106 91 Stockholm

Research

Structure and Mechanism of Solute Carrier (SLC) Transporters

Secondary transporters are the targets for many therapeutics and they often play a major role in drug pharmacokinetics. Understanding the mechanisms by which secondary transporters shuttle and move ions, drugs, and natural compounds across membranes is of fundamental importance. Because of the technical difficulties in working with membrane proteins our structural understanding is very limited. The goal of my research is to investigate the alternating access-mechanism of solute transporters which are critical to cell homeostasis and their dysfunction is associated with human diseases, such as cancer and cardiovascular heart disease. To achieve this we combine crystal structures with biochemical and biophysical techniques. These goals are facilitated by the development of novel methods in membrane protein overexpression, purification and crystallization.

 

Group members

Magnus Claesson, Lab Manager

Qie Kuang, Postdoc

Emmanuel Nji, Postdoc

Yurie Chatzikyriakidou, PhD Student

Pascal Meier, PhD Student

Abdul Aziz Qureshi, PhD Student

Povilas Uzdavinys, PhD Student

Iven Winkelmann, PhD Student

 

Selected Publications

  • Uzdavinys P, Coincon C, Nji E, Ndi M, Winkelman I, von Ballmoos C, Drew D. Dissecting the proton transport pathway in electrogenic Na+/H+ antiporters.Proc Natl Acad Sci U S A. (2017) Feb 14;114(7):E1101-E1110.
  • Drew D, Boudker O.Shared Molecular Mechanisms of Membrane Transporters. Annual Reviews in Biochemistry. (2016). Mar 21 85(1).                             
  • Coincon M, Uzdavinys P, Nji E, Dotson D.L, Winkelmann I, Abdul-Hussein S, Cameron A.D, Beckstein O, Drew D. Crystal structures reveal the molecular basis of ion-translocation in sodium/proton antiporters. Nature Structure & Molecular Biology. (2016) Mar:23(3):248-55.

  • Nomura N, Verdon G, Kang HJ, Shimamura T, Nomura Y, Sonoda Y, Hussien S, Qureshi A.A, Coincon M, Sato Y, Abe H, Nakada-Nakura Y, Hino T, Arakawa T, Kusano-Arai O, Iwanari H, Unno H, Murata T, Kobayashi T, Hamakubo T, Kasahara M, Iwata S, Drew D. Structure and mechanism of the mammalian fructose transporter GLUT5. Nature. (2015) 526(7573):397-401

  • Lee C, Kang HJ, von Ballmoos C, Newstead S, Uzdavinys P, Dotson D, Iwata S, Beckstein O, Cameron A.D, Drew D. A two-domain elevator mechanism for sodium/proton antiport.  Nature. (2013) Sept 26:501(7468):573-7.

  • Hu NJ, Iwata S, Cameron A.D, Drew D. Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT. Nature. (2011). Oct 3:478:408-11.

  • Sonoda Y, Newstead S, Hu N, Alguel Y, l Nji E, Beis K, Yashiro S, Lee C, Leung J, Cameron A.D, Byrne B, Iwata S, Drew DBenchmarking membrane protein thermostability for improving throughput of high-resolution X-ray structures. Structure. (2011). Jan 12:19(1):17-25.

  • Drew D, Newstead S, Sonoda, Y, Kim, H, von Heijne G, Iwata, S. GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae. Nature Protocols. (2008) 3(5):784-98. 

  • Newstead S, Kim H, von Heijne G, Iwata S, Drew D. High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in S. cerevisiaeProc Natl Acad Sci U S A. (2007) 104(35):13936-41. 

  • Drew D, Lerch M, Slotbboom D, Kunji, E, de Gier JW. Optimizing membrane protein overexpression and purification using GFP-fusions. Nature Methods. (2006) 3(4):303-313. 

Funding Sources

EMBO Young Investigator Program
Swedish Research council
The Knut and Alice Wallenberg Foundation
The Wenner-Gren Foundation
 

Files

  •  CV (127 Kb)

Last updated: September 20, 2017

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