Wide Field Microscop Cell Observer

An inverted microscope that is suitable for fixed samples, 3- and 4D live cell imaging (simultaneously at dual channels), bleaching techniques and microinjection experiments. The system has a climate hood for temperature and CO2 control and a heating stage for variable sized culture chambers. In addition there is a femtojet microinjection equipment from Eppendorf. The UV-light source is a Lambda DG-4 illumination system with external excitation filters. There are two digital cameras, AxioCam MRc with an extra emission filter wheel and a Roper Cascade 1K. The latter is coupled to a dual viewer for simultaneous detection of two channels. An external shutter for brightfield recording enables very fast acquisition of both fluorescence and bright field. Additional triggering signals can be added and steered by a TTL unit.

The following lenses are available:
EC-Plan-Neofluar 10x/0.3 Ph1
Plan-Neofluar 16x/0.5 Imm
EC-Plan-Neofluar 40x/ 0.75 Ph2
LCI Plan-Neofluar 63x/1.3 IMM corr (water or glycerol) Ph3
63x/1.4 oil DIC

The system has two optional soft wares for acquisition and image processing, Axiovision and Slide book.
A separate work station with Axiovision, Slide book and AutoQuant (for deconvolution) is available nearby.