Instruments

This inverted microscope is suitable for live cell imaging in 3 and 4D, bleaching (FRAP), photo activation techniques and spectral analyses. It is equipped with a diod laser 405 nm, an Argon laser with 458/488/514 nm lines, DPSS 561 nm and HeNe 633 nm laser. The instrument has a spectral detector (GaAsp 32-channels) for acquisition of Lambda Stacks and subsequent digital separation into component dyes, three additional PMTs, two for confocal detection and one for non-confocal bright field images. The spectral detection unit is adjustable within 390-750 nm. The instrument can be used for FCS and RICS. Standard equipment for ocular observation of reflected light is present as well as a heating stage and an incubator for controlled temperature and CO2-concentration.

The following lenses are available:

• Plan-Apo 10x/0.45
• Plan-Apo 20x/0.8
• Plan-Apo 40x/1.3 Oil
• Plan-Apo 63x/1.4 Oil
• LD C-Apo 63x/1.15 W corr

The system is controlled by the ZEN black 2011 software.

For image processing a separate workstation with identical software is available.

The Nikon AXR is a point scanning confocal built around the inverted Ti2E microscope, with additional resolution provided by the NSPARC APD array detector, which improves signal-to-noise and extends resolution (ISM). The system is equipped for live imaging.

Laser units

• LUD-S6 and Omicron laser nest 405 / 445 / 488 / 514 / 561 / 638/ 730

Detector units

• DUX with 3 GaAsP (2 tunable) and 1 IR-specific GaAsP PMT detectors
• NSPARC array detector of 25 APDs with down to 100 nm lateral x 300 nm axial resolution

Objective Lenses

This inverted microscope is suitable for live cell imaging in 3 and 4D, bleaching (FRAP), photo activation techniques and spectral analyses. It is equipped with solid state laser lines of 405 nm, 488 nm, 561 nm and 633 nm. The instrument has three highly sensitive GaAsP detectors of which one is an Airyscan detector. With the Airyscan techniqe you gain 1.7× higher resolution in all three dimensions. Standard equipment for ocular observation of reflected light is present as well as a heating stage and an incubator for controlled temperature and CO2-concentration.

The following lenses are available:

• EC plan-Neofluar 10x/0.3
• Plan-Apo 20x/0.8
• LD LCI Plan-Apo 25x/0.8 Imm Corr DIC
• Plan-Apo 63x/1.40 Oil DIC
• C-Apo 63x/1.20 W Corr

The system is controlled by the ZEN blue 2.1 software.

For image processing a separate workstation with ZEN software is available.

This inverted microscope is designed for TIRF applications but is also suitable for live cell imaging in 2D. It is equipped with an OPSL laser 488nm- and a DPSS 561 nm- laser. For acquisition, a CCD camera (Axiocam MRm) is used.  Standard equipment for ocular observation of reflected light is present as well as a heating stage for controlled temperature.

The following lenses are available:

• Plan-Apo 20x/0.6
• Plan-Apo 63x/1.46 Oil korr
• Plan-Fluar 100x/1.45 Oil

The system is controlled by the ZEN blue 2011 software.For image processing a separate workstation with identical software is available.

An inverted microscope that is suitable for fixed samples, 3- and 4D live cell imaging (simultaneously at dual channels), and microinjection experiments. The system has a climate hood for temperature and CO2 control and a heating stage for variable sized culture chambers. In addition there is a femtojet microinjection equipment from Eppendorf. The UV-light source is a Lambda DG-4 illumination system with external excitation filters. There are two digital cameras, AxioCam MRc with an extra emission filter wheel and a Roper Cascade 1K. The latter is coupled to a dual viewer for simultaneous detection of two channels. An external shutter for brightfield recording enables very fast acquisition of both fluorescence and bright field. Additional triggering signals can be added and steered by a TTL unit.

The following lenses are available:

• EC-Plan-Neofluar 10x/0.3 Ph1
• Plan-Neofluar 16x/0.5 Imm
• EC-Plan-Neofluar 40x/ 0.75 Ph2
• LCI Plan-Neofluar 63x/1.3 IMM corr (water or glycerol) Ph3
• Plan-Apochromate 63x/1.4 oil DIC

The system is controlled by the software Slide book for acquisition and image processing.

A separate work station with Slide book is available nearby.

An inverted microscope suitable for colour photos of histological staining in bright field mode as well as for fluorescent samples. The microscope is equipped with a colour camera, and a mercury lamp and filters for UV (Dapi), blue light (FITC/GFP) and green light (TRITC/Ds Red). Some of the objective lenses are specially designed for imaging through plastic and thick specimens (by modulation contrast).

The following lenses are available:

• N-Plan 10x/0.25 Ph1
• C-Plan L 20x/0.3 Ph1
• N-Plan L 20x/0.4 corr LMC (for modulation contrast)
• N-Plan L 40x/0.55 corr LMC (for modulation contrast)
• HCX PL APO 63x/1.32 oil Ph3

The microscope is a TECNAI G2 Spirit Bio TWIN (FEI Company) 120 kV with a Tungsten filament. It is a high contrast, general purpose instrument that is specifically suited for low contrast samples. It enables exploration of the native state of low contrast, beam sensitive biological specimens, or other soft materials like polymers. It has a BioTWIN low-background single-tilt holder and a Gatan 70° cryo transfer holder. There are two digital cameras: a SIS MegaView III (in-column) and a Gatan US 1000P (under the column).