Methods in Molecular Life Science
You will learn about various methods in life sciences, ranging from paleogenomics to transcriptomics to cryo-EM. You will also conduct a lab exercise that is a whole small study in yeast genomics.
This course is based on a comprehensive wet-lab exercise in yeast-covering genetic screening, next-generation sequencing, bioinformatics analyses and interpretation of results. Besides, this course has ten lectures on methods in life sciences - these lectures have been selected to be relatively complementary to other content at the MTLS programme.
The course lectures cover methods in molecular life sciences, with special emphasis on model systems and transcriptomics. Lectures could cover these areas: permafrost paleogenomics, cryo-EM, single-cell approaches in microbiology, genetic analysis and mutagenesis in yeast, fruit flies as a genetic model system, gene modifications in cells and animals, and experimental and computational transcriptomics. Besides the lectures, the yeast genomics exercise is an important part of the course. Here the students will screen for yeast cells with spontaneous mutations that allow them to grow on an ordinarily toxic medium. The students will then extract the DNA, prepare libraries for next-generation sequencing, and sequence the libraries on Illumina instruments. Finally, the students will perform advanced bioinformatics analyses to identify the mutations that originally allowed their yeast cells to grow in the toxic medium.
The course consists of ten class-room lectures and the yeast genomics exercise described above. The exercise will cover around eight afternoons of wet-lab exercise instances and three afternoons of bioinformatics exercise instances. Participation in the yeast genomics exercise is mandatory.
The examination will be a 3-hour written exam, and will test the students’ theoretical understanding of the ten lectures and the yeast genomics exercise.
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