Methods in Molecular Life Science
7 credits cr.
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You will learn about various methods in life sciences, ranging from paleogenomics to transcriptomics to cryo-EM. You will also conduct a lab exercise that is a whole small study in yeast genomics.
This course is based on a comprehensive wet-lab exercise in yeast-covering genetic screening, next-generation sequencing, bioinformatics analyses and interpretation of results. Besides, this course has ten lectures on methods in life sciences - these lectures have been selected to be relatively complementary to other content at the MTLS programme.
This course is part of the joint Master’s programme in Molecular techniques in life science, together with KTH and KI. The course is not open for application to students outside of the programme.
The freestanding course is Methods and Concepts in Molecular Life Sciences, 15 credits.
The course lectures cover methods in molecular life sciences, with special emphasis on model systems and transcriptomics. Lectures could cover these areas: permafrost paleogenomics, cryo-EM, single-cell approaches in microbiology, genetic analysis and mutagenesis in yeast, fruit flies as a genetic model system, gene modifications in cells and animals, and experimental and computational transcriptomics. Besides the lectures, the yeast genomics exercise is an important part of the course. Here the students will screen for yeast cells with spontaneous mutations that allow them to grow on an ordinarily toxic medium. The students will then extract the DNA, prepare libraries for next-generation sequencing, and sequence the libraries on Illumina instruments. Finally, the students will perform advanced bioinformatics analyses to identify the mutations that originally allowed their yeast cells to grow in the toxic medium.
The course consists of ten class-room lectures and the yeast genomics exercise described above. The exercise will cover around eight afternoons of wet-lab exercise instances and three afternoons of bioinformatics exercise instances. Participation in the yeast genomics exercise is mandatory.
The examination will be a 3-hour written exam, and will test the students’ theoretical understanding of the ten lectures and the yeast genomics exercise.
Tel: +46 73 7121558
ScheduleThe schedule will be available no later than one month before the start of the course. We do not recommend print-outs as changes can occur. At the start of the course, your department will advise where you can find your schedule during the course.
Course literatureNote that the course literature can be changed up to two months before the start of the course.
No mandatory course book.
ContactStudent officeStudy counsellor (advanced level)