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Analytical Chemistry, Advanced Separation Methods

This course focuses on the analytical chain and its components, describing the way in which the analytical chemist works and the most important methods in organic analytical chemistry. Including procedures going from relevant sampling, to sample treatment and instrumental analysis, and finally data treatment and presentation of results.   

The analytical chain

The course has a major focus on chromatographic methods, as well as treating sampling, sample clean-up/compound isolation, mass spectrometry, data treatment/statistical analysis, method development and oral and written presentation. It is the first course in the Master Program in Analytical Chemistry and a foundation for further specialization to become an analytical chemist, combining experimental method development with deeper theoretical understanding of important analytical processes.

  • Course structure

    The course consist of lectures, workshops, laboratory project work, oral and written presentation of scientific work within the following subjects in the chain of chemical analysis:

    • Sampling: Particulate Phase and Gaseous Phase Sampling, Filter and Adsorbent Sampling, Solid Phase Micro Extraction (SPME) and Denuder Technique.
    • Sample Treatment: Liquid-Liquid Extraction, Soxhlet Extraction, Ultrasonic Assisted Extraction, Accelerated Solvent Extraction (ASE) and Solid Phase Extraction (SPE).
    • Chromatography: Gas Chromatography, Liquid Chromatography (Including Reversed- and Normal Phase LC, Ion Chromatography, Size Exclusion Chromatography), Preparative Chromatography, Multidimensional Techniques and Method Development.
    • Mass spectrometry: Focus on GC/MS with Ionisation Techniques and Fragmentaion, with an introduction to LC/MS and MS/MS.

    Project work
    Literature and Experimental Projects - Project Planning, Risk Assessment, Method Development, Experimental Work, Oral presentation and Report Writing, Documentation and Good Laboratory Practice (GLP), Method validation.

    Refresher of basic statistics for analytical chemists - Exploratory Data Analysis, Regression and Correlation Analysis, Statistical Inference, t‐test, F‐test, Method Evaluation and Validation.

    Literature Project
    In this project students work together in a team to perform a literature study of a specific task. This includes searching scientific literature, compiling a report on the subject and presenting the subject in a seminar. A large part of these literature projects gives the groups a background to the two later experimental projects. The report is also peer reviewed before handed in for evaluation.

    Experimental Work
    Together in a team students solve two analytical tasks. In these projects you get to apply your knowledge acquired from the theoretical part of the course. The group (with feedback from the assistants) plans the projects and presents project plans (including a time schedule and work plans in order to complete the tasks within the stipulated time) before the projects starts.

    For each of the two experimental projects the task will be to prepare, perform and report a complete analytical procedure, based on the techniques presented during the course to solve the given tasks. This includes retrieval, compilation and use of scientific literature to plan the projects with respect to sampling, sample preparation, separation, identification, quantification using the analytical equipment available in the course labs, and perform statistical evaluation of the analytical data. You shall also present the projects and their results in two seminars and three written reports including a background of the analytes' chemical and toxicological properties.


    Theory, 8 ETCS
    Lab, 7 ECTS

    Teaching format

    The theoretical part includes lectures, homework assignments, group work, peer-review and seminars.

    Lab projects and content described above.


    In order to pass the course you have to pass the written examination (i.e. mark E or higher) as well as pass the oral and written presentations of the three projects.

    The literature project is graded “Not passed” or “Passed”.

    The two following experimental projects are graded using a scale with three grades (“Not passed”, “Passed”, “Well passed”) based on the performance during the projects and on the seminars as well as the written reports.

    The highest grade on the projects give a one grade increase on the total grade of the course.


    Nicole Pamme

  • Schedule

    The schedule will be available no later than one month before the start of the course. We do not recommend print-outs as changes can occur. At the start of the course, your department will advise where you can find your schedule during the course.

    Preliminary schedule, please watch for updates

  • Course literature

    Note that the course literature can be changed up to two months before the start of the course.

    The following books, together with lecture handouts and additional compendia handed out during the course, are the recommended literature for the course Advanced Separation Methods (KA7006).

    The literature reading guides are given for these selected books. All books are available at with a delivery time of approximately a week.

    Chromatography: Concepts and Contrasts
    James M Miller
    Wiley, 2009, ISBN: 9780470530252

    Gas Chromatography and Mass Spectrometry – A Practical Guide
    David Sparkman, Zelda Penton & Fulton Kitson
    Academic Press, 2011, ISBN: 9780123736284  (also available as e-book)  8


    Mass Spectrometry – Principles and Applications 3rd Ed    
    Edmond de Hoffman & Vincent Strobant
    Wiley, 2007, ISBN: 9780470033111  (also available as e-book)
    (Also used on the master course in mass spectrometry)

    Statistics and Chemometrics for Analytical Chemistry  6th Ed
    James Miller & Jane Miller
    Pearson, 2018, ISBN: 9781292186719  (also available as e-book)

  • Course reports

  • Contact

    Course coordinator and examiner
    Chemistry Section & Student Affairs Office