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Advanced Methods in Biochemistry

  • 7.5 credits

The course provides a thorough overview of modern biochemical and molecular biological methods, as well as available resources for gaining knowledge about these methods.

The course provides a thorough overview of modern biochemical and molecular biological methods, as well as available resources for gaining knowledge about these methods.

The techniques treated include pre-protein expression / purification methods, such as

  • cell disruption / fractionation
  • recombinant DNA technology
  • electrophoresis
  • mass spectrometry
  • microarrays.

Furthermore, data management and presentation of data is included

  • Course structure

    After completing the course, the student should be able to:

    • explain the principles behind biochemical methods
    • the strengths and weaknesses of these methods
    • explain the results obtained using these methods
    • be able to design experiments to investigate different biochemical issues by using and combining different techniques
    • be able to work according to “good laboratory practice” (GLP)
    • demonstrate proficiency in analyzing, interpreting and discussing results
    • present such data in both in writing and orally within given time frames

    Teaching format

    • Lectures and seminars on the theory of biochemical methods.
    • Laboratory training in biochemical methods
    • Seminars

    Assessment

    • Written exam
    • Home assignments
    • Lab reports

    Examiner

    Jan-Willem de Gier
    E-mail:       Jan-Willem.deGier@dbb.su.se

  • Course literature

    Note that the course literature can be changed up to two months before the start of the course.

    Book + course materials:

    • "Principles  and  Techniques  of  Biochemistry  and  Molecular  Biology  (8thedtion)".  Edited  by Andreas Hofmannn and Samuel Clokie (Cambridge University Press).
    • Hand-outs, articles etc. will be provided.
  • Contact

    Examiner

    Jan-Willem de Gier
    E-mail:       Jan-Willem.deGier@dbb.su.se

    Chemistry Section & Student Affairs Office:

    Office:        Chemical Practice Laboratory M345
    E-mail:       chemistry@su.se

    http://www.kemi.su.se/english