Ongoing projects*

  • FRET-FISH: combining DNA FISH with FLIM-FRET for quantification of chromosome X inactivation in mouse embryonic fibroblasts. Dept. of Physiology and Pharmacology, Karolinska Institutet. 
  • Multicolor cell labeling for solving the clonal structure of murine embryo neural crest. Dept. of Medical Biochemistry and Biophysics, Karolinska Institutet. 
    • In this project, we will experimentally target clonal structures of the cranial neural crest cells and will study their reparative plasticity as well as underlying signaling mechanisms that can be considered as new therapeutic targets. At the single cell and clonal levels, we will systematically explore the spectrum of signals integrating the growing oral and craniofacial complex. 
  • The effect of temperature on the bumblebee brain. Dept. of Zoology, Stockholm University. 
    • The aim of the project is to understand if and how the information carried by neurons in the bumblebee brain is affected by temperature. The findings will have consequences for understanding how increasing environmental temperatures, such as those that are occurring as a result of climate change, will affect the function of insect nervous systems and behavior.
  • Evaluation of autofluorescence life-time imaging to observe differences in biological samples. Dept. of Ecology, Environment and Plant Sciences, Stockholm University. Sample: Arabidopsis thaliana

 

 

 

 

  • Imaging Candida albicans infection
    in the mouse kidney. Dept. of Molecular Biosciences, Stockholm University

 

 

 

 

 

 

 

 

Finished projects*

  • Live monitoring of cellular dynamics during skeletal development and maintenance. Department of Physiology and Pharmacology, Karolinska Institutet. 
  • Second harmonic generation imaging of collagen gels. Department of Micro and Nanosystems, KTH Royal Institute of Technology, Sample: hydrogel based on type I collagen
  • Determining the effect of Piezo1 activation on membrane tension in cancer cells. Department of Medical Cell Biology, Uppsala University. Samples: MDA-MB-231 breast cancer cells
  • Real-time visualisation of human stem cells during neuronal differentiation. Department of Organismal Biology, Uppsala University. Samples: Human embryonic stem cells

*All in vivo projects are performed in compliance with ethics permits approved by the local ethics committee.

 

Publications 

Blood-brain barrier-restricted translocation of Toxoplasma gondii from cortical capillaries. Gabriela C. Olivera, Emily C. Ross, Christiane Peuckert, Antonio Barragan. Elife. 2021;10:e69182.doi: 10.7554/eLife.69182. 

Three Dimensional Microvascularized Tissue Models by Laser-Based Cavitation Molding of Collagen. Alessandro Enrico, Dimitrios Voulgaris, Rebecca Östmans, Naveen Sundaravadivel, Lucille Moutaux, Aurélie Cordier, Frank Niklaus, Anna Herland, and Göran Stemme. Adv Mater. 2022;e2109823.doi: 10.1002/adma.202109823