Group Östlund Farrants

Group Östlund Farrants

Chromatin and Chromatin Remodelling:
The function of ATP-dependent chromatin remodelling complexes

The organisation of DNA into chromatin, with its smallest unit the nucleosome, plays a role in gene regulation, replication, recombination, and repair. The chromatin forms an obstacle for a number of protein factors that need access to the DNA. Several protein complexes have been identified that change the chromatin structure and make the DNA more accessible. These complexes regulate the structure either by acetylation, phosphorylation and methylation of the histone proteins in the nucleosome, or by changing the histone-DNA contacts using ATP. The latter complexes are called ATP-dependent chomatin remodelling complexes and several different types exist.

The central question in the projects presented is to understand the function of different ATP-dependent chromatin remodelling complexes in mammalian cells. We focus on two different types of complex, the SWI/SNF family of complexes, and the ISWI-complex B-WICH. The SWI/SNF chromatin remodelling complexes are involved mainly in transcription, whereas the ISWI-complexes are involved in replication, nucleosome spacing and transcription.

Research project

SWI/SNF complexes

Chromatin Remodelling in RNA polymerase II Transcription. SWI/SNF complexes Main investigator: Stefani Böhm

Research project

ISWI-complexes

Chromtatin Remodelling and RNA polymerase I and III Transcripion. ISWI-complexes Main investigators: Fatemeh Sadeghifar and Anna Vintermist

Selected publications

Vintermist. A., Böhm. S., Sadeghifar. F., Louvet. E., Mansén. A., Percipalle. P., Ostlund Farrants. A.K. The chromatin remodelling complex B-WICH changes the chromatin structure and recruits histone acetyl-transferases to active rRNA genes. PLoS One. 2011 Apr 29;6(4):e19184. PMID: 21559432

Ryme. J., Asp. P., Böhm. S., Cavellán. E., Farrants Ostlund. A.K. Variations in the composition of mammalian SWI/SNF chromatin remodelling complexes. J Cell Biochem. 2009 Oct 15;108(3):565-76. PMID: 19650111

Tyagi. A., Ryme. J., Brodin. D., Ostlund Farrants. A.K, Visa, N. SWI/SNF associates with nascent pre-mRNPs and regulates alternative pre-mRNA processing. PLoS Genet. 2009 May;5(5):e1000470. Epub 2009 May 8. PMID: 19424417

Botelho. SC., Tyagi, A., Hessle, V., Farrants, AK., Visa. N. The association of Brahma with the Balbiani ring 1 gene of Chironomus tentans studied by immunoelectron microscopy and chromatin immunoprecipitation. Insect Mol Biol. 2008 Sep;17(5):505-13. PMID: 18754808

Obrdlik. A., Kukalev. A., Louvet. E., Farrants. A.K, Caputo. L., Percipalle. P. The histone acetyltransferase PCAF associates with actin and hnRNP U for RNA polymerase II transcription. Mol Cell Biol. 2008 Oct;28(20):6342-57. Epub 2008 Aug 18.

Anki Ö F

 

Ann-Kristin Östlund Farrants, Professor

Visiting address:
Svante Arrhenius väg 20C
Room F448

Postal address:
Stockholm University
Department of Molecular Biosciences,
The Wenner-Gren Institute
SE-106 91 Stockholm

Telephone: +46 8 16 4097
E-mail: anki.ostlund@su.se

Group members

Yuan Guo
Jaclyn Quin
Kanwal Tariq