Before starting a project

The first step is to make sure that the Zeiss Xradia Versa 520 is the right machine for your research purpose.  Below are some factors to take into consideration.

X-ray source

• Microfocus tungsten X-ray source

• Energy range: 10.0 - 160.0 keV

Detector

• Scintillator-based camera with 4 different objectives: 0.4x, 4x, 20x and 40x

• CCD: 2048 x 2048 pixels, 13.5 µm pixel size

• Field of view (FOV): adjustable between approximately 0.6 mm to 60 mm. Stitching is possible to extend the FOV further.

• Effective pixel sizes adjustable between approximately 0.1 µm and 34 µm (if no binning). It depends on the objective (optical magnification) and the distances between the source, sample and detector (geometrical magnification).

• Spatial resolution: approximately 0.7 µm in 2D, about 1 µm in 3D. 

Sample preparation

• Up to 14 automatic-loading sample holders

• Mechanical loading stages can be mounted consulting with SUBIC staff

• Staining may be recommended for imaging small and complex soft tissues. SUBIC cannot supply staining chemicals at the moment.