We are interested in factors affecting the sensitivity of eukaryotic cells to ionising radiation. We work with various cell types that we irradiate with different doses and dose rates of ionising radiation. We have sources for both low and high-LET radiation. We analyse cytogenetic effects (chromosomal aberrations and micronuclei), DNA repair (γH2AX foci-kinetics, comet assay) and cell survival. In addition, we are also interested in the mechanisms underlying individual radiosensitivity and methods of biological dosimetry.
Keywords
Radiation sensitivity, biological dosimetry, chromosomal aberrations, DNA damage
Selected publications
Plodowska, M, Lopez-Riego M, Akuwudike P, Sobota D, Filipek M, Kłosowski M, Kaźmierczak U, Brzozowska B, Baliga A, Lisowska H, Braziewicz J, Olko P, Lundholm L, Wojcik A. Small is beautiful: low activity alpha and gamma sources for small-scale radiation protection research experiments. Int J Radiat Biol. 2021, DOI:10.1080/09553002.2021.1867925
Olofsson D, Cheng L, Fernández RB, Płódowska M, Riego ML, Akuwudike P, Lisowska H, Lundholm L, Wojcik A. Biological effectiveness of very high gamma dose rate and its implication for radiological protection. Radiat Environ Biophys. 59: 451–460. 2020. doi: 10.1007/s00411-020-00852-z
Brzozowska-Wardecka B, Tartas A, Wojcik A. Monte Carlo modeling of DNA lesions and chromosomal aberrations induced by mixed beams of alpha particles and X-rays. Frontiers in Physics, 8:567864. doi: 10.3389/fphy.2020.567864.
Cheng L, Brzozowska-Wardecka B, Lisowska H, Wojcik A, Lundholm L. Impact of ATM and DNA-PK inhibition on gene expression and individual response of human lymphocytes to mixed beams of alpha particles and X-rays. Cancers, 11(12), 2013, 2019; https://doi.org/10.3390/cancers11122013
Gałecki M, Tartas A, Szymanek A, Sims E, Lundholm L, Sollazzo A, Cheng L, Fujishima Y, Yoshida MA, Żygierewicz J, Wojcik A, Brzozowska-Wardecka B. Precision of scoring radiation-induced chromosomal aberrations and micronuclei by unexperienced scorers. Int J Radiat Biol. 95:1251-1258, 2020. doi: 10.1080/09553002.2019.1625462
Wojcik A, Schenk L, Enghag M, Lundegard I, Arvanitis, L, Haglund K, Hamza K. Educating about radiation risks in high schools: towards an improved understanding of the complexity of low dose radiation health effects. Radiat Environ Biophys, 58: 13-20. 2019. doi:10.1007/s00411-018-0763-4
Cheng L, Brzozowska B, Sollazzo A, Lundholm L, Lisowska L, Haghdoost S, Wojcik A. Simultaneous induction of dispersed and clustered DNA lesions compromises DNA damage response in human peripheral blood lymphocytes. PLoS ONE 13(10): e0204068, 2018, doi.org/10.1371/journal.pone.0204068.
Chiba M, Monzen S, Iwaya C, Kashiwagi Y, Yamada S, Hosokawa A, Mariya Y, Nakamura T, Wojcik A. Serum miR-375-3p increase in mice exposed to a high dose of ionizing radiation. Scientific Reports 8: 1302, 2018, doi:10.1038/s41598-018-19763-7.
Sollazzo A, Brzozowska B, Cheng L, Lundholm L, Scherthan H, Wojcik A. Live Dynamics of 53BP1 Foci Following Simultaneous Induction of Clustered and Dispersed DNA Damage in U2OS Cells. Int J Mol Sci. 2018 Feb 8;19(2). pii: E519. doi: 10.3390/ijms19020519.
Eriksson M, Hååg P, Brzozowska B, Lipka M, Lisowska H, Lewensohn R, Wojcik A, Viktorsson K, Lundholm L. Analysis of Chromatin Opening in Heterochromatic Non-Small Cell Lung Cancer Tumor-Initiating Cells in Relation to DNA-Damaging Antitumor Treatment. Int J Radiat Oncol Biol Phys. 2018 Jan 1;100(1):174-187. doi: 10.1016/j.ijrobp.2017.09.033.
Brzozowska B, Gałecki M, Tartas A, Ginter J, Kaźmierczak U, Lundholm L. Freeware tool for analysing numbers and sizes of cell colonies. Radiat Environ Biophys. 2019 Mar;58(1):109-117. doi: 10.1007/s00411-018-00772-z.
Wojcik A, Hamza K, Lundegård I, Enghag M, Haglund K, Arvanitis L, Schenk L. Educating about radiation risks in high schools: towards improved public understanding of the complexity of low-dose radiation health effects. Radiat Environ Biophys. 2019 Mar;58(1):13-20. doi: 10.1007/s00411-018-0763-4.
Wojcik A, Bouffler S, Hauptmann M, Rajaraman P. Considerations on the use of the terms radiosensitivity and radiosusceptibility. J Radiol Prot. 2018 Sep;38(3):N25-N29. doi: 10.1088/1361-6498/aacb03.
Rajaraman P, Hauptmann M, Bouffler S, Wojcik A. Human individual radiation sensitivity and prospects for prediction. Ann ICRP. 2018 Oct;47(3-4):126-141. doi: 10.1177/0146645318764091.
