Profiles

Britt-Marie Sjöberg

Britt-Marie Sjöberg

Professor

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Works at Department of Biochemistry and Biophysics
Telephone 08-16 41 50
Email britt-marie.sjoberg@dbb.su.se
Visiting address Svante Arrhenius väg 16
Room A355
Postal address Institutionen för biokemi och biofysik 106 91 Stockholm

Research

The ribonucleotide reductase enzyme family

Organisms store their genetic information in DNA, and the enzyme ribonucleotide reductase (RNR) catalyses the reaction that provides new DNA building blocks. RNR is therefore a prerequisite in all living cells, and also regulates cellular replication and proliferation. Some antiproliferative drugs (e.g. hydroxyurea and gemcitabine) that are used clinically today are specific inhibitors of RNR.

There are currently three different classes of RNRs (I, II, and III) that differ in reactivity towards oxygen, cofactor requirement, and quaternary structure. Our database RNRdb (http://rnrdb.pfitmap.org) also distinguishes several RNR subclasses. Despite the striking differences all RNRs appear to have a common evolutionary origin.

Current projects are:

  • developing novel antibiotics towards RNRs in pathogenic microorganisms
  • exploring the potential use of RNRs as environmental biomarkers
  • understanding the emergence of ribonucleotide reduction in the RNP (RNA+protein) world
  • understanding the evolution of extant RNRs

 

Group members

Margareta Sahlin, Docent

Daniel Lundin, Researcher

Inna Rozman Grinberg, Postdoc

 

Selected Publications

  • Johansson, R., Rao Jonna, V., Kumar, R., Nayeri, N., Lundin, D., Sjöberg, B.-M., Hofer, A. & Logan, D.T. Structural mechanism of allosteric activity regulation in a ribonucleotide reductase with double ATP cones, Structure (2016) 24: 906-917.
  • Crona, M., Hofer, A., Astorga-Wells, J., Sjöberg, B.-M. & Tholander, F. Biochemical characterization of the split class II ribonucleotide reductase from Pseudomonas aeruginosa. PLoS One (2015) 10: e0134293
  • Rao Jonna, V., Crona, M., Rofougaran, R., Lundin, D., Johansson, S., Brännström, K., Sjöberg, B.-M. & Hofer, A. Diversity in overall activity regulation of ribonucleotide reductase. J Biol Chem (2015) 290: 17339-17348.
  • Aurelius, O., Johansson, R., Bågenholm, V., Lundin, D., Tholander, F., Balhuizen, A., Beck, T., Sahlin, M., Sjöberg, B.-M., Mulliez, E. & Logan D.T. The crystal structure of Thermotoga maritima class III ribonucleotide reductase lacks a radical cysteine pre-positioned in the active site. PLoS One (2015) 10: e0128199.
  • Lundin, D., Berggren, G., Logan, D.T. & Sjöberg, B.-M. The origin and evolution of ribonucleotide reduction. Life (Basel) (2015) 5, 604-36.
  • Berggren, G., Duraffourg, N., Sahlin, M. & Sjöberg, B.-M. Semiquinone-induced maturation of Bacillus anthracis ribonucleotide reductase by a superoxide intermediate. J Biol Chem (2014) 289: 31940-9.
  • Crona, M., Avesson, L., Sahlin, M., Lundin, D., Hinas, A., Klose, R., Söderbom, F & Sjöberg, B.-M. A rare combination of ribonucleotide reductases in the social amoeba Dictyostelium discoideum. J Biol Chem (2013) 288:8198-208.
  • Tholander, F. & Sjöberg, B.-M. Discovery of antimicrobial ribonucleotide reductase inhibitors by screening in microwell format. Proc Natl Acad Sci USA (2012) 109: 9798-803. (covered by senior science writer R. Mukhopadhyay in Wild Types Blog, ASBMB Today 2012/06/04)
  • Lundin, D., Poole, A.M., Sjöberg, B.-M. & Högbom, M. Use of structural phylogenetic networks for classification of the ferritin-like superfamily. J Biol Chem (2012) 287: 20565-75.
  • Hofer, A., Crona, M., Logan, D.T. & Sjöberg, B.-M. DNA building blocks: keeping control of manufacture. Critical Rev Biochem Mol Biol (2012) 47: 50-63.
  • Lundin D, Gribaldo S, Torrents E, Sjöberg B-M & Poole AM. Ribonucleotide reduction - horizontal transfer of a required function spans all three domains. BMC Evolutionary Biology (2010) 10:383 (selected “highly accessed” by the editors of BMC Evol Biol)
  • Sjöberg B-M. A never-ending story. Science (2010) 329: 1475-1476.

 

Funding Sources

The Swedish Research Council, The Swedish Cancer Foundation, The Wenner-Gren Foundations

Last updated: July 25, 2017

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