Creation of different cell types from an identical DNA sequence is one of the most remarkable properties of genomes, and a fundamental question in developmental biology. Control of transcription establishes the gene expression programs that define a cell and directs cell differentiation. It is thereby critical for embryo development. A mechanistic view of transcriptional regulation is not only necessary for recognition of how specific cell states are established and epigenetically maintained, but also for understanding how its misregulation leads to disease. The Drosophila embryo allows us to dissect these mechanisms in vivo by a set of tools that are not readily available elsewhere. Molecular, genetic, and genomic approaches are used to identify the molecular mechanisms by which tissue-specific gene expression is controlled in vivo.

 

Keywords

transcription, co-regulators, genome-wide, chromatin, epigenetics, embryo patterning

 

Selected publications

Boija A, Mahat DB, Zare A, Holmqvist PH, Philip P, Meyers DJ, Cole PA, Lis JT, Stenberg P, Mannervik M (2017) CBP Regulates Recruitment and Release of Promoter-Proximal RNA Polymerase II. Molecular Cell, 68, 491-503.

Yeung K, Boija A, Karlsson E, Holmqvist PH, Tsatskis Y, Nisoli I, Yap D, Lorzadeh A, Moksa M, Hirst M, Aparicio S, Fanto M, Stenberg P, Mannervik M, McNeill H (2017) Atrophin controls developmental signaling pathways via interactions with Trithorax-like. eLife, 6, pii: e23084.

Boija A and Mannervik M (2016). Initiation of diverse epigenetic states during nuclear programming of the Drosophila body plan. Proc Natl Acad Sci U S A, 113(31):8735-40.

Dahlberg O, Shilkova O, Tang M, Holmqvist PH and M. Mannervik (2015). P-TEFb, the Super Elongation Complex and Mediator Regulate a Subset of Non-paused Genes during Early Drosophila Embryo Development. PLoS Genetics, 11(2):e1004971.

Nègre, N., Brown, C.D., Ma, L., Bristow, C.A., Miller, S.W., Wagner, U., Kheradpour, P., Eaton, M.L., Loriaux, P., Sealfon, R., Li, Z., Ishii, H., Spokony, R.F., Chen, J., Hwang, L., Cheng, C., Auburn, R.P., Davis, M.B., Domanus, M., Shah, P.K., Morrison, C.A., Zieba, J., Suchy, S., Senderowicz, L., Victorsen, A., Bild, N.A., Grundstad, A.J., Hanley, D., MacAlpine, D.M., Mannervik, M., Venken, K., Bellen, H., White, R., Gerstein, M., Russell, S., Grossman, R.L., Ren, B., Posakony, J.W., Kellis, M., & White, K.P. (2011). A cis-regulatory map of the Drosophila genome. Nature. 471(7339):527-31

 

 

 

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(Link to PubMed)