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Stefan NordlundProfessor of Biochemistry

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Contact

Name and title: Stefan Nordlund Professor of Biochemistry

stefan@dbb.su.se

08-16 29 32

Workplace: Department of Biochemistry and Biophysics

Visiting address

Room C 470

Svante Arrhenius väg 16

Postal address

Institutionen för biokemi och biofysik

106 91 Stockholm

Research group

Stefan Nordlund's research group

Nitrogen metabolism plays a central role in any organism. We are interested in more specifically identifying the difference in the pattern of proteins expressed when the bacterium fixes nitrogen compared with conditions when nitrogenase is not synthesized.

Research

Regulation of nitrogen fixation in photosynthetic bacteria

This project is a collaboration with Agneta Norén
Nitrogen metabolism plays a central role in any organism and our studies concern the process leading to conversion of atmospheric nitrogen, N2, to forms that can be used in the cell through ammonia assimilation. We have in particular focussed on the regulation of these processes in the beautiful photosynthetic bacterium Rhodospirillum rubrum. Over the years we have narrowed down our efforts to understanding of the metabolic regulation of nitrogenase, the enzyme catalyzing nitrogen fixation.

This fascinating control system involves reversible covalent modification of one of the two components of nitrogenase, the Fe-protein. The modified protein is inactive. We are aiming at identifying the signal(s) to the two enzymes DRAT, which catalyzes the modification, and DRAG catalyzing the removal of the modification, an ADP-ribose.

We are in addition interested in more specifically identify the difference in the protein pattern when the bacterium fixes nitrogen compared with conditions when nitrogenase is not synthesized, i.e. when there is sufficient usabele nitrogen compounds available.

The methods we employ span from basic biochemistry and molecular biology to proteomics.

Selected Publications

Selao, T.S., Branca, R., Chae, P.S., Lehtiö, J., Gellman, S.H., Rasmussen, S.G.F., Nordlund, S. and Norén, A. (2011) Identification of chromatophore membrane protein complexes formed under different nitrogen availability conditions in Rhodospirillum rubrum. J. Proteome. 10:2703-2714.
Berthold, C., Wang, H., Nordlund, S., and Högbom, H. (2009) Mechanism of ADP-ribosylation removal revealed by the structure and ligand complexes of thedimanganese mono-ADP-ribosylhydrolase DraG. Proc. Natl. Acad. Sci. USA 106:14247-14252.
Wang, H., Franke, C.C., Nordlund, S. and Norén, A. (2005). Reversible membrane association of dinitrogenase reductase activating glycohydrolase in the regulation of nitrogenase activity in Rhodospirillum rubrum; dependence on GlnJ and AmtB1. FEMS Microbiol. Lett. 253:273-279.
Nordlund, S. and Ludden, P.W. (2004) “Post-translational regulation of nitrogenase in photsynthetic bacteria”, in “Genetics and regulation of nitrogen fixing bacteria”, (Klipp, W., Masephol, B., Gallon, J.R. and Newton, W.E. eds.) Kluwer Academic Press, the Netherlands. 175-196.

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