Research project CRYSTAL-X - Uncovering hidden structures in protein crystallization with coherent X-rays
This project studies protein crystallization in ferritin microdroplets. Using X-ray Photon Correlation Spectroscopy and facilities like ESRF and Eu-XFEL, it investigates hidden intermediate states and water's role in the crystallization process.
This project aims to unravel the mysteries of protein crystallization, a key process for understanding protein functions and dynamics. Focusing on ferritin solutions in microdroplets, we investigate anomalous crystallization pathways using X-ray Photon Correlation Spectroscopy (XPCS) to study the dynamics of proteins and amorphous precursors. We also examine the role of water structuring near protein surfaces and its impact on desolvation, a critical step influencing crystal formation. Leveraging advanced facilities like ESRF and Eu-XFEL, this research explores non-classical crystallization to uncover new insights into protein behavior.
Project members
Project managers
Anita Girelli
Postdoktor

Members
Foivos Perakis
Associate professor
