The research primarily focuses on understanding how mammalian cells maintain genomic stability and by which mechanisms low doses of gamma radiation, UV radiation and certain chemicals destabilise the genome. The focus is on nucleotide pool imbalance and oxidation. There are strong inter-individual variations among humans regarding the maintenance of genomic stability and response to toxic agents which could be due to the genetic background and life style factors. Understanding the underlying mechanisms that make some individuals sensitive to UV and ionizing radiation or chemicals is very important for estimating individual risk of cancer, diabetes complications, cardiovascular and other age-related diseases.

 

Haghdoost group
Haghdoost group

 

Keywords: Oxidative stress, genomic instability, nucleotide pool imbalance, MTH1, individual radiation sensitivity, DNA damage, DNA repair

 

Selected publications

D. Danielsson K. Brehwens, M. Halle, M. Marczyk, A. Sollazzo, J. Polanska, E. Munck-Wikland, A. Wojcik, S. Haghdoost, Influence of genetic background and stress response on risk of mandibular osteoradionecrosis after radiotherapy of head and neck cancer. Accepted Article in journal of ”Head and Neck”, Nov. 2014, doi: 10.1002/ijc.23903

A. Fotouhi, W.W. Hagos, M. Ilic, A. Wojcik, M. Harms-Ringdahl, F. de Gruijl, L. Mullenders, J.G. Jansen, S. Haghdoost, Analysis of mutant frequencies and mutation spectra in hMTH1 knockdown TK6 cells exposed to UV radiation, Mutation research, 751-752 (2013) 8-14.

S. Osterman Golkar, S. Czene, A. Gokarakonda, S. Haghdoost, Intracellular deoxyribonucleotide pool imbalance and DNA damage in cells treated with hydroxyurea, an inhibitor of ribonucleotide reductase, Mutagenesis, 28 (2013) 653-660.

R. Yentrapalli, O. Azimzadeh, A. Sriharshan, K. Malinowsky, J. Merl, A. Wojcik, M. Harms-Ringdahl, M.J. Atkinson, K.F. Becker, S. Haghdoost, S. Tapio, The PI3K/Akt/mTOR pathway is implicated in the premature senescence of primary human endothelial cells exposed to chronic radiation, PloS one, 8 (2013) e70024.

A. Fotouhi, S. Skiold, S. Shakeri-Manesh, S. Osterman-Golkar, A. Wojcik, D. Jenssen, M. Harms-Ringdahl, S. Haghdoost, Reduction of 8-oxodGTP in the nucleotide pool by hMTH1 leads to reduction in mutations in the human lymphoblastoid cell line TK6 exposed to UVA, Mutation research, 715 (2011) 13-18.

S. Skiold, I. Naslund, K. Brehwens, A. Andersson, P. Wersall, E. Lidbrink, M. Harms-Ringdahl, A. Wojcik, S. Haghdoost, Radiation-induced stress response in peripheral blood of breast cancer patients differs between patients with severe acute skin reactions and patients with no side effects to radiotherapy, Mutation research, 756 (2013) 152-157.