Proteins in eukaryotic cells
15 credits cr.
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The course deals with the expression of proteins and silencing of gene expression in eukaryotic cells.
In addition, post-translational modification, intracellular sorting and transport of protein in eukaryotic cells are treated. Furthermore, methods for gene editing, studies of protein distribution and dynamics, as well as protein-protein interactions in vitro and in living cells.
A large part of the course consists of laboratory work, where students work with various current research issues in protein biochemistry.
After completing the course, the student is expected to:
- be able to explain the expression system of genes in mammalian cells and how gene expression can be edited or silenced in eukaryotic cells, as well as reflect on human responsibility for how these methods are used.
- explain methods can be used to study protein distribution and interactions in mammalian cells, including in living cells.
- be able to experimentally express and analyze proteins in eukaryotic cells
- show insight into current research issues in protein biochemistry.
- be able to make assessments and discuss societal and ethical aspects of protein biochemistry.
Theory 5 ECTS
Practical laboratory work 10 ECTS
- Group projects
Theory, written exam
Lab, written lab reports, written and oral presentations