In this study I highlight the importance of the Reconquista and the Way of St. James in the Iberian Peninsula. Even though the historic events are well known, we do not know to what extent the Muslim expansion and posterior Reconquista affected the Iberian population. In this study, I generated data from 24 individuals from two medieval Christian necropolises in the north of Iberia spanning from the XI to XIII centuries. The results indicate that there were no signs of the North-African genetic component that most of the modern Spanish have in the medieval individuals analysed here. The individuals could be descendants from the first settlers who came from the Christian territories in the north of the Iberian Peninsula, suggesting that Muslims and Christians did not mix in the first stages of the Reconquista, at least not in the North of the Iberian Peninsula. The importance the Way of Saint James had during the Middle Ages is also stressed as I found what could possibly be a pilgrim from England that died before completing his pilgrimage.