Methods in Molecular Life Science
You will learn about various methods in life sciences, ranging from paleogenomics to functional genomics to cryo-EM. You will also conduct a lab exercise that is a whole small study in yeast genomics.
This course is based on a comprehensive wet-lab exercise in yeast - covering genetic screening, next-generation sequencing, bioinformatics analyses and interpretation of results. Besides, this course has ten lectures on methods in life sciences - these lectures have been selected to be relatively complementary to other content at the MTLS programme.
This course is part of the joint Master’s programme in Molecular techniques in life science, together with KTH and KI. The course is not open for application to students outside of the programme.
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Course structure
The course lectures cover methods in molecular life sciences, with special emphasis on model systems and transcriptomics. Lectures could cover these areas: permafrost paleogenomics, cryo-EM, functional genomics, genetic analysis and mutagenesis in yeast, and the use of plants, nematodes and fruit flies as model systems. Besides the lectures, the yeast genomics exercise is an important part of the course. Here the students will screen for yeast cells with spontaneous mutations that allow them to grow on an ordinarily toxic medium. The students will then extract the DNA, and libraries for NGS will be prepared and sequenced on an Illumina instrument. Finally, the students will perform advanced bioinformatics analyses to identify the mutations that originally allowed their yeast cells to grow in the toxic medium, and will write a report on their entire study.
Teaching format
The course consists of ten class-room lectures and the yeast genomics exercise described above. The exercise will cover around six afternoons of wet-lab exercise instances and five mornings of bioinformatics exercise instances. Participation in the yeast wet-lab exercises is mandatory.
Assessment
The examination will be a 3-hour written exam, and will test the students’ theoretical understanding of the ten lectures and the yeast genomics exercise.
Examiner
Marc Friedländer
E-mail: marc.friedlander@scilifelab.se
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Schedule
The schedule will be available no later than one month before the start of the course. We do not recommend print-outs as changes can occur. At the start of the course, your department will advise where you can find your schedule during the course. -
Course literature
Note that the course literature can be changed up to two months before the start of the course.
No mandatory course book.
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Course reports
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Contact
Student office- Visiting address
Arrheniuslaboratory, room E315
Svante Arrhenius väg 20C
- Office hours
Mon-Thur: 13.00-15.00
- Irregular office hours
Closed Thursday November 21!
Study counsellor for advanced level
- Visiting address
Arrheniuslaboratoriet, room E315
Svante Arrhenius väg 20 C
- Office hours
By request
