Stockholm university

Research group Group Friedländer

Our group applies computational and omics methods to study mammalian transcriptome regulation, with special emphasis on microRNAs.

The Friedländer group applies beyond-state-of-the-art computational and Omics methods to address fundamental questions in RNA biology. Our focus is on quantitatively describing and functionally characterizing mammalian transcriptomes, and methods include next-generation sequencing of single and pooled cells, as well as development of new algorithms and experimental methods.

Of particular interest to us are microRNAs: 22 nucleotide long RNA molecules that regulate protein-coding genes in all multi-cellular animals. Since they confer regulation on the majority of human genes, it is not surprising that microRNAs are involved in numerous biological processes – including diseases such as metabolic and neurodegenerative diseases and cancer. Even though microRNAs have been systematically studied for more than twenty years, fundamental questions regarding their evolution, biogenesis and function remain unanswered.

We study microRNA function by profiling these regulators and their gene targets in the single cells where the interactions between them occur. From the measurements we infer copy-per-cell numbers for the transcripts, and we develop mathematical models to describe the kinetics of regulation. For this purpose, we apply single-cell sequencing and screening methods. Recently, we have developed a method to detect microRNA targeting in single cells, using an editing-capable TRIBE fusion protein. To address evolutionary aspects of microRNAs, we compare microRNAs between representatives of all major animal groups, using the highly curated MirGeneDB database – that we have contributed to developing.

Our recent research includes studies of microRNA biogenesis in living cells, discovering authentic RNA molecules in ancient samples from the Siberian permafrost, and profiling microRNAs in astronaut blood in collaboration with NASA (see our Publications).

Our group is part of Stockholm University but is located at the SciLifeLab hub. We work closely with the research groups of Claudia Kutter and Vicent Pelechano (both at the Karolinska Institute) – for instance having joint lab meetings and journal clubs. Our research is funded by the Swedish Research Council (VR), the Swedish Strategic Research Areas (SFO), the Swedish Cancer Foundation (Cancerfonden) and a starting grant from the European Research Council (ERC).
 

Group members

Group managers

Marc Friedländer

University Lecturer

Department of Molecular Biosciences, The Wenner-Gren Institute
Marc Friedländer

Members

Ioannis Alexopoulos

Student

Department of Molecular Biosciences, The Wenner-Gren Institute

Tiago Figueiredo

Student

Department of Molecular Biosciences, Wenner-Grens institute

Daniela Almeida

Guest researcher

Department of Molecular Biosciences, The Wenner-Gren Institute

Morteza Aslanzadeh

PhD student

Department of Molecular Biosciences, The Wenner-Gren Institute
Morteza Aslanzadeh

Inna Biryukova

Forskare

Department of Molecular Biosciences, The Wenner-Gren Institute

Panagiotis Kalogeropoulos

PhD student

Department of Molecular Biosciences, The Wenner-Gren Institute

Vaishnovi Sekar

PhD student

Department of Molecular Biosciences, The Wenner-Gren Institute
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Research projects

Publications

News