Research project Argonaute TRIBE

We have developed a new method to detect microRNA targeting in single cells using the so-called TRIBE approach, where an RNA-binding protein of interest is fused to an editing-capable protein domain. In effect, Argonaute-loaded microRNAs guide the fusion protein to its targets, and the editing domain leaves irreversible marks that can be detected using single-cell RNA sequencing. We foresee that Argonaute TRIBE will be useful to the wider research community – both as a new single-cell method, but also as a bulk method that circumvents the need for laborious immunoprecipitation and high-quality antibodies.