By: Jayanta Chaudhuri, Memorial Sloan Kettering Cancer Center, New York, USA

Title: AID'ing Recombination and Repair

Immunoglobulin heavy chain class switch recombination (CSR) is essential for robust B cell-mediated immunity. CSR requires AID, a DNA deaminase that converts cytidines to uridines in switch (S) region DNA that precede each constant region gene segment. DNA deamination potentiates a cascade, leading to the generation of DNA double-strand breaks (DSBs) at S regions. End-joining of DSBs between acceptor and donor S regions completes CSR. We will discuss the mechanism by which non-coding RNA recruits AID specifically to S regions and how AID participates not only in DNA deamination, but also in the conversion of deaminated residues into DSBs.

Host: Eva Severinson