• Iris Söll and Giselbert Hauptmann: Multicolored Visualization of Transcript Distributions in Drosophila Embryos.

• Iris Söll and Giselbert Hauptmann: Manual and Automated Whole-Mount In Situ Hybridization for Systematic Gene Expression Analysis in Embryonic Zebrafish Forebrain.

• Jamie Ian Morrison: Fluorescent In-Situ Hybridization Detection of MicroRNAs in Newt Tissue Cryosections.

• Giselbert Hauptmann, Gilbert Lauter and Iris Söll: Application of Alkaline Phosphatase-Mediated Azo Dye Staining for Dual Fluorescent In Situ Hybridization in Zebrafish.


In Situ Hybridization Methods

This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.


In Situ Hybridization Methods
In Situ Hybridization Methods

Fore more information: